Human methylome studies SRP533334 Track Settings
Study title: Methylation patterns of the nasal epigenome of hospitalized SARS-CoV-2 positive patients reveal insights into molecular mechanisms of COVID-19
SRA: SRP533334
GEO: not found
Pubmed: not found
Experiment
Label
Methylation
Coverage
HMRs
HMR size
AMRs
AMR size
PMDs
PMD size
Conversion
Details
SRX26114832
Nasopharyngeal Swab
0.699
15.1
69842
1161.8
2096
972.6
3163
13520.6
0.992
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 399, COVID, Mild", "age": "55", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114833
Nasopharyngeal Swab
0.741
12.0
70087
1225.5
1056
981.2
3449
16372.7
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 13, COVID, Mild", "age": "61", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114834
Nasopharyngeal Swab
0.706
12.5
69837
1179.9
1127
996.0
2651
13460.3
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 94, COVID, Mild", "age": "48", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114835
Nasopharyngeal Swab
0.724
12.5
41435
1141.0
1843
959.2
2527
9976.6
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 117, COVID, Mild", "age": "49", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114836
Nasopharyngeal Swab
0.718
11.9
39007
1198.4
1308
955.5
2290
9909.6
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 153, COVID, Mild", "age": "51", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114837
Nasopharyngeal Swab
0.652
22.1
56155
968.6
11046
981.3
3492
13608.7
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 194, COVID, Mild", "age": "70", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114838
Nasopharyngeal Swab
0.698
17.7
94990
1115.2
1323
1065.1
3072
14615.2
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 418, COVID, Mild", "age": "71", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114839
Nasopharyngeal Swab
0.729
18.8
57781
981.4
4168
918.6
3662
11287.9
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 334, COVID, Mild", "age": "11", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114840
Nasopharyngeal Swab
0.739
14.6
50178
1037.3
4589
904.8
3257
11913.2
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 171, COVID, Mild", "age": "15", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114841
Nasopharyngeal Swab
0.739
14.1
56832
1012.2
3003
863.4
3411
13224.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 80, COVID, Mild", "age": "59", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114842
Nasopharyngeal Swab
0.698
13.8
49271
1021.1
5633
959.3
2995
10933.9
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 176, COVID, Mild", "age": "27", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114843
Nasopharyngeal Swab
0.697
13.4
81594
1100.0
687
957.3
3025
15276.8
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 241, COVID, Mild", "age": "63", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114844
Nasopharyngeal Swab
0.764
10.4
55502
1006.8
393
905.7
3118
10794.1
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 18, COVID, Mild", "age": "66", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114845
Nasopharyngeal Swab
0.747
8.2
39495
1182.3
639
863.0
1684
15767.5
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 86, COVID, Mild", "age": "15", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114846
Nasopharyngeal Swab
0.676
8.3
37730
1223.8
1825
872.4
1465
16527.0
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 96, COVID, Mild", "age": "15", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114847
Nasopharyngeal Swab
0.616
20.2
62219
868.4
4751
939.0
3805
10519.7
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 214, COVID, Mild", "age": "16", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114848
Nasopharyngeal Swab
0.713
15.1
52398
990.8
4010
909.4
3486
11094.6
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 189, COVID, Mild", "age": "21", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114849
Nasopharyngeal Swab
0.659
19.6
49343
991.5
10067
974.6
3265
10747.0
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 221, COVID, Mild", "age": "23", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114850
Nasopharyngeal Swab
0.727
17.2
49213
1047.8
2646
937.7
2884
10655.0
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 193, COVID, Mild", "age": "28", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114851
Nasopharyngeal Swab
0.640
19.0
48263
1022.1
12413
998.3
3623
11815.9
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 317, COVID, Mild", "age": "28", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114852
Nasopharyngeal Swab
0.762
12.2
57841
984.6
680
894.8
3660
10955.3
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 110, COVID, Mild", "age": "19", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114853
Nasopharyngeal Swab
0.750
12.3
48574
1070.5
5010
925.5
3426
12367.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 202, COVID, Mild", "age": "29", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114854
Nasopharyngeal Swab
0.732
12.8
59537
1206.5
2467
941.2
2784
14298.3
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 73, COVID, Mild", "age": "43", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114855
Nasopharyngeal Swab
0.733
5.1
38380
1327.4
521
956.9
902
27102.0
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 324, COVID, Mild", "age": "44", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114856
Nasopharyngeal Swab
0.752
21.7
52822
1048.1
6320
944.0
3386
11836.3
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 7, COVID, Mild", "age": "57", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114857
Nasopharyngeal Swab
0.715
13.7
42914
1113.8
5860
918.9
3171
11147.4
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 97, COVID, Mild", "age": "32", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114858
Nasopharyngeal Swab
0.724
9.5
37129
1245.0
973
849.6
2935
9123.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 57, COVID, Mild", "age": "34", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114859
Nasopharyngeal Swab
0.673
15.8
68464
860.1
4175
974.9
3682
8980.3
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 248, COVID, Mild", "age": "37", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114860
Nasopharyngeal Swab
0.752
16.1
62685
937.3
1292
880.1
3848
9630.8
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 416, COVID, Mild", "age": "37", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114861
Nasopharyngeal Swab
0.683
10.0
42721
1126.7
1879
901.6
2667
9971.0
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 161, COVID, Mild", "age": "28", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114862
Nasopharyngeal Swab
0.744
17.8
53980
1018.2
3212
848.4
3223
11906.4
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 27, COVID, Severe", "age": "38", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114863
Nasopharyngeal Swab
0.759
16.9
70404
905.1
983
872.0
3866
11161.0
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 162, COVID, Mild", "age": "41", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114864
Nasopharyngeal Swab
0.746
9.8
44079
1136.0
2005
898.1
3177
10993.0
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 48, COVID, Mild", "age": "41", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114865
Nasopharyngeal Swab
0.694
16.2
76715
1163.4
1373
1041.5
3191
14698.7
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 42, COVID, Severe", "age": "69", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114866
Nasopharyngeal Swab
0.741
16.0
60030
979.1
2469
939.2
3129
11825.1
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 165, COVID, Mild", "age": "77", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114867
Nasopharyngeal Swab
0.714
6.9
45165
1198.9
346
859.8
1915
15185.0
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 2, COVID, Mild", "age": "62", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114868
Nasopharyngeal Swab
0.753
11.5
55839
1019.6
726
896.8
3622
10920.6
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 210, COVID, Mild", "age": "27", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114869
Nasopharyngeal Swab
0.757
11.8
45332
1097.0
4052
887.8
3150
12340.3
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 17, COVID, Mild", "age": "23", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114870
Nasopharyngeal Swab
0.725
12.1
41815
1125.5
2823
886.6
2972
10978.3
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 53, COVID, Mild", "age": "25", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114871
Nasopharyngeal Swab
0.653
15.6
43293
1052.7
4215
985.7
2809
10434.8
0.992
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 200, COVID, Mild", "age": "23", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114872
Nasopharyngeal Swab
0.759
14.2
51831
1035.4
3001
850.2
3523
11003.0
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 12, COVID, Mild", "age": "26", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114873
Nasopharyngeal Swab
0.733
14.4
45410
1094.4
6578
934.4
3018
11452.6
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 44, COVID, Mild", "age": "42", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114874
Nasopharyngeal Swab
0.717
14.9
78505
1155.1
1238
902.3
3521
15116.8
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 420, COVID, Mild", "age": "43", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114875
Nasopharyngeal Swab
0.735
10.4
43782
1137.4
1112
862.3
3254
9754.8
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 26, COVID, Mild", "age": "52", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114876
Nasopharyngeal Swab
0.747
9.1
50196
1116.0
505
897.9
3361
12011.0
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 14, COVID, Mild", "age": "53", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114877
Nasopharyngeal Swab
0.712
15.7
48222
1041.7
1481
887.7
2942
8658.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 290, COVID, Severe", "age": "54", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114878
Nasopharyngeal Swab
0.754
13.4
49479
1060.0
3145
867.6
3300
10169.0
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 62, COVID, Mild", "age": "55", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114879
Nasopharyngeal Swab
0.754
9.3
54008
1027.9
530
889.5
1868
19670.4
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 209, COVID, Mild", "age": "60", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114880
Nasopharyngeal Swab
0.711
15.3
78285
1173.9
1219
1053.9
3224
15721.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 38, COVID, Mild", "age": "32", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114881
Nasopharyngeal Swab
0.723
11.8
69360
1184.4
572
937.0
3169
15062.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 56, COVID, Mild", "age": "27", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114882
Nasopharyngeal Swab
0.742
14.1
53935
1020.8
2699
860.2
3779
10889.6
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 179, COVID, Mild", "age": "28", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114883
Nasopharyngeal Swab
0.738
13.2
42476
1090.7
2775
884.7
3429
10598.6
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 170, COVID, Mild", "age": "30", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114884
Nasopharyngeal Swab
0.727
11.3
56024
1007.6
852
940.5
3623
9390.6
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 144, COVID, Mild", "age": "32", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114885
Nasopharyngeal Swab
0.755
13.4
62120
963.7
740
882.9
3805
11110.8
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 361, COVID, Mild", "age": "33", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "White"}
SRX26114886
Nasopharyngeal Swab
0.736
11.3
50919
1058.1
2570
857.2
3441
11217.7
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 308, COVID, Mild", "age": "36", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114887
Nasopharyngeal Swab
0.708
5.6
36408
1353.0
379
967.8
1496
18373.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 78, COVID, Mild", "age": "37", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114888
Nasopharyngeal Swab
0.745
13.7
52165
1026.6
2726
860.3
3286
10375.7
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 173, COVID, Mild", "age": "43", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114889
Nasopharyngeal Swab
0.674
15.1
49738
1020.6
1229
940.4
2660
9585.9
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 259, COVID, Severe", "age": "66", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "male", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114890
Nasopharyngeal Swab
0.719
9.5
50016
1054.0
776
925.1
3374
10105.7
0.994
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 158, COVID, Mild", "age": "36", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
SRX26114891
Nasopharyngeal Swab
0.721
13.2
41584
1141.0
3236
954.2
2753
10191.9
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 164, COVID, Mild", "age": "43", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Other"}
SRX26114892
Nasopharyngeal Swab
0.698
13.6
47092
1092.8
6311
937.4
3141
13289.5
0.993
title: Whole Genome Bisulfite Sequencing of hospitalized SARS-CoV-2 positive patients; {"isolate": "Nasal, 306, COVID, Mild", "age": "43", "biomaterial_provider": "TMC", "collection_date": "2020-04/2020-08", "geo_loc_name": "USA: Missouri, Kansas City", "sex": "female", "tissue": "Nasopharyngeal swab", "race": "Black"}
Methods
All analysis was done using a bisulfite sequnecing data analysis
pipeline DNMTools
developed in the Smith lab at USC.
Mapping reads from bisulfite sequencing :
Bisulfite treated reads are mapped to the genomes with the
abismal
program. Input reads are
filtered by their quality, and adapter sequences in the 3' end of
reads are trimmed. This is done with cutadapt. Uniquely mapped reads
with mismatches/indels below given threshold are retained. For
pair-end reads, if the two mates overlap, the overlapping part of the
mate with lower quality is discarded. After mapping, we use the format
command in dnmtools to merge mates for paired-end reads. We use the
dnmtools uniq command to randomly select one from multiple reads
mapped exactly to the same location. Without random oligos as UMIs,
this is our best indication of PCR duplicates.
Estimating methylation levels :
After reads are mapped and filtered, the
dnmtools counts
command is used to obtain read
coverage and estimate methylation levels at individual cytosine
sites. We count the number of methylated reads (those containing a
C) and the number of unmethylated reads (those containing a T) at
each nucleotide in a mapped read that corresponds to a cytosine in
the reference genome. The methylation level of that cytosine is
estimated as the ratio of methylated to total reads covering that
cytosine. For cytosines in the symmetric CpG sequence context,
reads from the both strands are collapsed to give a single
estimate. Very rarely do the levels differ between strands
(typically only if there has been a substitution, as in a somatic
mutation), and this approach gives a better estimate.
Bisulfite conversion rate :
The bisulfite conversion rate for an experiment is estimated with
the dnmtools bsrate
command, which computes the
fraction of successfully converted nucleotides in reads (those
read out as Ts) among all nucleotides in the reads mapped that map
over cytosines in the reference genome. This is done either using
a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear
genome. In the latter case, only non-CpG sites are used. While
this latter approach can be impacted by non-CpG cytosine
methylation, in practice it never amounts to much.
Identifying hypomethylated regions (HMRs) :
In most mammalian cells, the majority of the genome has high
methylation, and regions of low methylation are typically the
interesting features. (This seems to be true for essentially all
healthy differentiated cell types, but not cells of very early
embryogenesis, various germ cells and precursors, and placental
lineage cells.) These are valleys of low methylation are called
hypomethylated regions (HMR) for historical reasons. To identify
the HMRs, we use the dnmtools hmr
command, which uses
a statistical model that accounts for both the methylation level
fluctations and the varying amounts of data available at each CpG
site.
Partially methylated domains :
Partially methylated domains are large genomic regions showing
partial methylation observed in immortalized cell lines and
cancerous cells. The pmd
program is used to identify
PMDs.
Allele-specific methylation :
Allele-Specific methylated regions refers to regions where the
parental allele is differentially methylated compared to the
maternal allele. The program allelic
is used to
compute allele-specific methylation score can be computed for each
CpG site by testing the linkage between methylation status of
adjacent reads, and the program amrfinder
is used to
identify regions with allele-specific methylation.
For more detailed description of the methods of each step, please
refer to
the DNMTools
documentation .
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