Human methylome studies SRP047086 Track Settings
Study title: A public-private-academic consortium, Genome-in-a-Bottle (GIAB), hosted by NIST to develop reference materials and standards for clinical sequencing
SRA: SRP047086
GEO: not found
Pubmed: not found
Experiment
Label
Methylation
Coverage
HMRs
HMR size
AMRs
AMR size
PMDs
PMD size
Conversion
Details
SRX9500354
B-Lymphocyte
0.530
3.2
8968
26584.8
66
1155.5
1245
1314538.5
0.996
title: Methylome capture of HG001 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500355
B-Lymphocyte
0.533
3.5
10568
25483.4
107
1058.7
1281
1285775.3
0.996
title: Methylome capture of HG001 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500356
B-Lymphocyte
0.669
1.5
25475
2988.4
11
5857.1
406
2788956.0
0.996
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500357
B-Lymphocyte
0.670
1.5
26287
2952.5
13
1454.5
422
2726983.3
0.996
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500358
B-Lymphocyte
0.669
7.9
41094
2659.5
389
949.8
755
1843249.1
0.997
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500359
B-Lymphocyte
0.671
5.6
37258
2739.4
185
936.3
732
1883680.3
0.997
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500360
B-Lymphocyte
0.672
2.3
28916
2900.8
22
1171.2
505
2435681.4
0.997
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500362
B-Lymphocyte
0.672
2.3
28887
2893.3
21
1202.7
478
2498748.6
0.997
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500363
B-Lymphocyte
0.672
8.7
41540
2528.5
594
896.8
775
1808053.0
0.997
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500364
B-Lymphocyte
0.674
8.3
40597
2510.3
615
914.4
765
1823411.3
0.997
title: Methylome capture of HG002 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500367
B-Lymphocyte
0.567
5.0
29364
14350.0
58
1033.3
1040
1498592.2
0.997
title: Methylome capture of HG003 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500368
B-Lymphocyte
0.569
4.6
28728
14353.1
51
1107.9
1016
1526177.3
0.997
title: Methylome capture of HG003 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500369
B-Lymphocyte
0.567
3.5
26511
14036.6
31
1179.8
942
1621886.2
0.997
title: Methylome capture of HG003 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500370
B-Lymphocyte
0.567
3.5
26228
13998.2
28
1087.1
955
1606390.5
0.997
title: Methylome capture of HG003 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500371
B-Lymphocyte
0.566
13.1
43103
11043.5
638
965.8
816
1691969.5
0.997
title: Methylome capture of HG003 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500374
B-Lymphocyte
0.570
12.9
43228
10961.5
683
973.0
868
1617367.8
0.997
title: Methylome capture of HG003 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500376
B-Lymphocyte
0.626
1.4
24789
4249.4
12
1310.2
600
2293032.5
0.997
title: Methylome capture of HG004 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500377
B-Lymphocyte
0.626
4.1
36320
6591.3
119
1390.2
864
1761817.6
0.997
title: Methylome capture of HG004 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500378
B-Lymphocyte
0.628
5.5
39070
6665.0
298
1198.4
921
1676530.3
0.997
title: Methylome capture of HG004 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500381
B-Lymphocyte
0.631
4.0
36603
6308.6
109
1502.7
863
1768774.7
0.997
title: Methylome capture of HG004 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500382
B-Lymphocyte
0.633
5.3
39134
6307.9
293
1234.7
896
1715750.6
0.997
title: Methylome capture of HG004 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500383
B-Lymphocyte
0.640
3.0
30858
5092.2
21
1157.0
839
1761640.0
0.996
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500385
B-Lymphocyte
0.640
3.1
31679
5115.9
22
1149.7
815
1801818.7
0.996
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500386
B-Lymphocyte
0.640
11.4
46632
5280.4
408
945.6
834
1704097.2
0.996
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500387
B-Lymphocyte
0.645
7.3
43602
5415.7
189
993.6
965
1567594.9
0.997
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500388
B-Lymphocyte
0.642
1.9
27829
4655.0
11
1520.3
708
1994275.7
0.996
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500389
B-Lymphocyte
0.643
2.0
27708
4652.1
7
1679.0
703
2002546.4
0.997
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500390
B-Lymphocyte
0.643
7.4
43110
5476.1
154
1004.4
1019
1512948.6
0.997
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500391
B-Lymphocyte
0.643
11.6
47350
5250.8
437
936.7
830
1701846.7
0.996
title: Methylome capture of HG005 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500392
B-Lymphocyte
0.659
2.1
26608
3643.6
10
1382.8
595
2248119.2
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500393
B-Lymphocyte
0.660
2.1
27367
3650.5
12
1383.4
623
2175619.1
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500394
B-Lymphocyte
0.660
7.6
39491
3180.7
163
1029.6
948
1617375.6
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500396
B-Lymphocyte
0.661
8.3
39566
3143.0
240
977.3
572
2174333.4
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500397
B-Lymphocyte
0.658
2.5
28581
3430.9
20
1219.5
625
2177621.1
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500398
B-Lymphocyte
0.659
2.6
29439
3457.3
23
1221.4
654
2108920.7
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500399
B-Lymphocyte
0.659
13.6
44610
3020.6
543
948.6
948
1600140.1
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500400
B-Lymphocyte
0.661
9.7
42428
2911.7
372
964.4
653
2020680.2
0.997
title: Methylome capture of HG006 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500401
B-Lymphocyte
0.666
3.9
31770
3254.6
63
1043.6
769
1888874.7
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500402
B-Lymphocyte
0.667
3.9
32948
3247.1
63
1148.4
819
1810086.4
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500403
B-Lymphocyte
0.667
19.0
48329
3095.5
1625
1060.2
603
2050446.6
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500404
B-Lymphocyte
0.666
8.5
40345
3025.8
622
1151.2
654
2008248.2
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500405
B-Lymphocyte
0.663
2.2
27323
3533.4
12
1507.9
617
2206329.0
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500407
B-Lymphocyte
0.664
2.2
27564
3484.9
12
1564.2
593
2249684.1
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500408
B-Lymphocyte
0.664
9.0
41497
3038.9
649
1101.8
675
1973997.0
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500409
B-Lymphocyte
0.669
14.2
46135
2999.2
1370
1071.1
575
2092353.8
0.997
title: Methylome capture of HG007 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500410
B-Lymphocyte
0.550
2.7
16638
10715.8
180
1084.9
877
1696513.2
0.986
title: Methylome capture of HG001 using TruSeq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500411
B-Lymphocyte
0.550
2.7
16601
10769.8
185
1110.2
906
1662577.8
0.986
title: Methylome capture of HG001 using TruSeq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500412
B-Lymphocyte
0.567
3.8
19575
10926.2
427
1077.4
947
1604743.2
0.986
title: Methylome capture of HG001 using TruSeq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500415
B-Lymphocyte
0.552
8.8
22168
15905.3
1043
1079.8
1120
1396194.0
0.987
title: Methylome capture of HG001 using TruSeq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500416
B-Lymphocyte
0.658
1.9
26789
2189.1
52
1177.6
35
198740.1
0.987
title: Methylome capture of HG002 using TruSeq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500418
B-Lymphocyte
0.659
1.9
26504
2187.7
48
1325.7
62
156990.6
0.987
title: Methylome capture of HG002 using TruSeq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500419
B-Lymphocyte
0.661
6.0
34189
1982.5
412
992.4
226
43440.6
0.987
title: Methylome capture of HG002 using TruSeq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500420
B-Lymphocyte
0.660
3.0
30366
2120.8
114
1103.2
70
80779.1
0.985
title: Methylome capture of HG002 using TruSeq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500421
B-Lymphocyte
0.660
3.1
29918
2122.8
123
1072.2
95
66785.7
0.985
title: Methylome capture of HG002 using TruSeq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500422
B-Lymphocyte
0.662
9.2
38414
1900.7
707
946.3
282
47414.4
0.985
title: Methylome capture of HG002 using TruSeq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500423
B-Lymphocyte
0.590
2.4
24186
5375.6
52
1380.9
587
2253232.4
0.985
title: Methylome capture of HG003 using TruSeq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500424
B-Lymphocyte
0.590
2.4
24177
5391.1
46
1441.7
558
2324301.3
0.986
title: Methylome capture of HG003 using TruSeq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500425
B-Lymphocyte
0.596
11.0
34074
8738.3
933
997.2
703
1930637.2
0.986
title: Methylome capture of HG003 using TruSeq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500426
B-Lymphocyte
0.594
3.7
25524
6202.2
197
1075.2
596
2206838.6
0.986
title: Methylome capture of HG003 using TruSeq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500427
B-Lymphocyte
0.594
3.7
26148
6274.4
192
1096.7
633
2115595.2
0.986
title: Methylome capture of HG003 using TruSeq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500429
B-Lymphocyte
0.592
7.5
31126
8873.7
556
1021.0
716
1917102.1
0.986
title: Methylome capture of HG003 using TruSeq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500432
B-Lymphocyte
0.630
5.7
31935
3889.4
735
1096.4
596
2245681.9
0.984
title: Methylome capture of HG004 using TruSeq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500433
B-Lymphocyte
0.628
3.6
28236
3541.9
351
1143.4
498
2523999.7
0.986
title: Methylome capture of HG004 using TruSeq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500434
B-Lymphocyte
0.629
3.6
27863
3542.2
365
1133.7
517
2471744.4
0.986
title: Methylome capture of HG004 using TruSeq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500435
B-Lymphocyte
0.631
10.2
36298
4314.2
1192
1104.8
380
2800212.9
0.986
title: Methylome capture of HG004 using TruSeq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500436
B-Lymphocyte
0.636
3.4
27816
3051.2
86
1159.2
540
2339456.2
0.986
title: Methylome capture of HG005 using TruSeq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500437
B-Lymphocyte
0.637
3.4
27298
3058.5
91
1182.0
529
2378195.4
0.986
title: Methylome capture of HG005 using TruSeq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500438
B-Lymphocyte
0.638
10.1
36050
3431.0
313
994.4
681
1985503.5
0.985
title: Methylome capture of HG005 using TruSeq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500440
B-Lymphocyte
0.636
2.4
26515
2936.6
56
1100.7
441
2642993.3
0.987
title: Methylome capture of HG005 using TruSeq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500441
B-Lymphocyte
0.637
2.4
25605
2917.0
54
1146.7
473
2549410.1
0.987
title: Methylome capture of HG005 using TruSeq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500442
B-Lymphocyte
0.639
7.9
34269
3368.8
268
1036.1
541
2270353.0
0.986
title: Methylome capture of HG005 using TruSeq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500445
B-Lymphocyte
0.653
17.3
41383
2096.2
512
1034.6
403
2588248.8
0.986
title: Methylome capture of HG006 using TruSeq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500446
B-Lymphocyte
0.652
6.5
32927
2137.9
246
1108.4
451
2578973.2
0.985
title: Methylome capture of HG006 using TruSeq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500447
B-Lymphocyte
0.652
6.5
33504
2136.8
258
1090.2
485
2481472.6
0.986
title: Methylome capture of HG006 using TruSeq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500448
B-Lymphocyte
0.656
5.0
31533
2197.1
168
1114.2
165
63297.5
0.985
title: Methylome capture of HG006 using TruSeq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500449
B-Lymphocyte
0.659
4.6
30380
2085.9
466
1076.1
112
47011.2
0.986
title: Methylome capture of HG007 using TruSeq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500451
B-Lymphocyte
0.660
4.6
29883
2109.4
484
1081.9
160
43001.7
0.986
title: Methylome capture of HG007 using TruSeq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500452
B-Lymphocyte
0.661
13.5
39895
1978.4
1259
1081.0
530
2270694.8
0.987
title: Methylome capture of HG007 using TruSeq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500455
B-Lymphocyte
0.661
5.3
31695
2058.3
608
1095.7
164
40814.3
0.986
title: Methylome capture of HG007 using TruSeq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500456
B-Lymphocyte
0.483
26.1
52937
12515.1
1680
1070.3
1520
1081477.9
0.996
title: Methylome capture of HG001 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500458
B-Lymphocyte
0.478
5.5
8621
34062.3
119
1040.3
1319
1229429.4
0.993
title: Methylome capture of HG001 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500459
B-Lymphocyte
0.483
9.5
25828
19249.2
777
1097.2
1357
1192051.5
0.994
title: Methylome capture of HG001 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500463
B-Lymphocyte
0.619
10.0
46395
5740.1
1020
1137.2
834
1738910.7
0.997
title: Methylome capture of HG004 using EMSeq with 100ng of input DNA; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500464
B-Lymphocyte
0.610
13.2
47658
5404.9
1729
1074.0
675
1920646.5
0.998
title: Methylome capture of HG004 using EMSeq with 100ng of input DNA; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500465
B-Lymphocyte
0.610
19.1
51263
5223.9
2368
1066.7
700
1883879.9
0.998
title: Methylome capture of HG004 using EMSeq with 100ng of input DNA; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500466
B-Lymphocyte
0.632
10.6
46982
5073.9
438
972.6
935
1576134.1
0.994
title: Methylome capture of HG005 using EMSeq with 10ng of input DNA; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500467
B-Lymphocyte
0.629
15.4
50660
4840.1
632
946.0
728
1814011.5
0.997
title: Methylome capture of HG005 using EMSeq with 50ng of input DNA; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500468
B-Lymphocyte
0.625
16.3
49521
4901.5
730
916.1
742
1786708.9
0.997
title: Methylome capture of HG005 using EMSeq with 100ng of input DNA; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500470
B-Lymphocyte
0.612
17.8
49523
4431.9
1345
906.4
724
1781193.2
0.998
title: Methylome capture of HG005 using EMSeq with 10ng of input DNA; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500471
B-Lymphocyte
0.620
16.1
50363
4551.1
1056
898.4
741
1768438.7
0.998
title: Methylome capture of HG005 using EMSeq with 50ng of input DNA; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500472
B-Lymphocyte
0.615
15.1
49030
4526.7
1021
917.9
727
1785949.4
0.998
title: Methylome capture of HG005 using EMSeq with 100ng of input DNA; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500473
B-Lymphocyte
0.650
13.7
45217
3027.5
663
930.7
634
1986570.8
0.993
title: Methylome capture of HG006 using EMSeq with 10ng of input DNA; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500475
B-Lymphocyte
0.651
12.0
44036
2767.0
537
950.6
599
2041280.0
0.996
title: Methylome capture of HG006 using EMSeq with 50ng of input DNA; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500476
B-Lymphocyte
0.648
12.9
43986
2776.3
593
968.1
592
2050100.2
0.997
title: Methylome capture of HG006 using EMSeq with 100ng of input DNA; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500478
B-Lymphocyte
0.639
26.7
49790
2988.5
1353
911.0
706
1831991.4
0.997
title: Methylome capture of HG006 using EMSeq with 10ng of input DNA; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500479
B-Lymphocyte
0.645
14.3
44836
2684.2
887
917.6
632
1956900.1
0.998
title: Methylome capture of HG006 using EMSeq with 50ng of input DNA; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500480
B-Lymphocyte
0.620
20.8
48620
2288.4
1360
895.3
880
1412335.7
0.995
title: Methylome capture of HG002 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500481
B-Lymphocyte
0.609
4.9
32809
2977.3
88
1033.2
422
2554908.7
0.992
title: Methylome capture of HG002 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500482
B-Lymphocyte
0.613
11.6
42154
2361.5
809
898.9
860
1445967.0
0.993
title: Methylome capture of HG002 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500483
B-Lymphocyte
0.535
14.9
43664
10153.5
778
946.0
770
1724984.6
0.996
title: Methylome capture of HG003 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500484
B-Lymphocyte
0.528
8.5
30957
12962.1
227
973.6
778
1771622.2
0.994
title: Methylome capture of HG003 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500485
B-Lymphocyte
0.534
8.7
34005
12310.7
363
960.5
751
1806539.7
0.994
title: Methylome capture of HG003 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500486
B-Lymphocyte
0.586
19.0
48167
5101.6
1426
1109.3
607
2035301.6
0.996
title: Methylome capture of HG004 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500487
B-Lymphocyte
0.577
5.8
36017
7309.0
207
1273.2
708
1951198.9
0.993
title: Methylome capture of HG004 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500488
B-Lymphocyte
0.581
7.6
38729
6468.6
522
1175.3
600
2101216.0
0.994
title: Methylome capture of HG004 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500489
B-Lymphocyte
0.591
14.3
46654
4385.6
466
957.0
634
1927076.1
0.997
title: Methylome capture of HG005 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500490
B-Lymphocyte
0.583
8.2
39210
5245.1
183
1076.9
631
1979754.9
0.992
title: Methylome capture of HG005 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500491
B-Lymphocyte
0.587
10.6
43148
4608.7
456
927.2
637
1888803.7
0.993
title: Methylome capture of HG005 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500493
B-Lymphocyte
0.614
13.5
42705
2516.7
483
960.1
557
2084298.1
0.996
title: Methylome capture of HG006 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500494
B-Lymphocyte
0.611
15.0
44135
2612.3
531
954.4
639
1917653.0
0.995
title: Methylome capture of HG006 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500495
B-Lymphocyte
0.618
14.8
42234
2548.1
1124
1056.0
631
1904478.2
0.995
title: Methylome capture of HG007 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500496
B-Lymphocyte
0.615
23.0
46156
2747.6
1613
1045.4
605
1950334.4
0.993
title: Methylome capture of HG007 using TrueMethyl oxidative bisulite sequencing, mockBS replicate; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500497
B-Lymphocyte
0.487
22.8
49976
13200.4
1397
1120.6
1516
1082058.1
0.995
title: Methylome capture of HG001 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500498
B-Lymphocyte
0.479
4.8
6642
38174.6
83
1171.1
1286
1252106.1
0.994
title: Methylome capture of HG001 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500499
B-Lymphocyte
0.489
11.1
30447
17454.8
846
1060.7
1288
1204905.1
0.994
title: Methylome capture of HG001 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500500
B-Lymphocyte
0.614
5.3
33470
2646.9
79
1104.8
481
2386499.4
0.994
title: Methylome capture of HG002 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500501
B-Lymphocyte
0.622
10.5
41475
2242.6
525
1001.7
461
2311456.5
0.995
title: Methylome capture of HG002 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500502
B-Lymphocyte
0.610
4.6
31670
2950.7
61
1121.6
579
2182365.8
0.992
title: Methylome capture of HG002 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500505
B-Lymphocyte
0.617
9.5
40100
2362.2
509
1024.0
507
2185792.6
0.994
title: Methylome capture of HG002 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500506
B-Lymphocyte
0.527
4.2
20514
16526.9
33
1052.0
925
1619293.1
0.994
title: Methylome capture of HG003 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500507
B-Lymphocyte
0.537
5.9
27218
14416.4
100
1159.9
909
1622546.2
0.995
title: Methylome capture of HG003 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500508
B-Lymphocyte
0.527
4.6
20773
16043.3
39
1108.1
889
1651055.6
0.995
title: Methylome capture of HG003 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500509
B-Lymphocyte
0.536
7.2
29913
13159.6
194
1034.6
694
1901009.9
0.995
title: Methylome capture of HG003 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500510
B-Lymphocyte
0.576
2.6
27411
7075.7
18
1203.2
656
2118680.5
0.995
title: Methylome capture of HG004 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500511
B-Lymphocyte
0.586
10.8
42907
5596.6
795
1176.0
560
2132429.8
0.996
title: Methylome capture of HG004 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500512
B-Lymphocyte
0.576
5.2
34358
7342.0
149
1409.9
643
2078475.9
0.994
title: Methylome capture of HG004 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500513
B-Lymphocyte
0.584
7.4
39048
6463.6
489
1198.2
590
2120250.2
0.994
title: Methylome capture of HG004 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500514
B-Lymphocyte
0.586
5.0
34096
5826.8
53
1291.1
733
1862330.6
0.995
title: Methylome capture of HG005 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500516
B-Lymphocyte
0.593
7.7
39345
4836.4
196
1068.3
638
1976600.3
0.996
title: Methylome capture of HG005 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500517
B-Lymphocyte
0.582
3.7
31938
6400.1
26
1221.9
668
1994401.8
0.994
title: Methylome capture of HG005 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500518
B-Lymphocyte
0.587
9.7
41531
4487.7
354
960.1
670
1904855.8
0.994
title: Methylome capture of HG005 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500519
B-Lymphocyte
0.615
15.8
43479
2618.8
490
935.3
559
2071523.0
0.995
title: Methylome capture of HG006 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500520
B-Lymphocyte
0.607
4.5
31392
3462.4
56
1046.5
676
2025887.9
0.994
title: Methylome capture of HG006 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500521
B-Lymphocyte
0.613
4.7
31288
3180.2
69
1174.1
682
2010824.8
0.994
title: Methylome capture of HG006 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500522
B-Lymphocyte
0.618
12.0
40944
2466.0
918
1075.0
657
1860825.2
0.994
title: Methylome capture of HG007 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500523
B-Lymphocyte
0.610
4.5
30484
3244.9
65
1100.5
495
2390477.2
0.992
title: Methylome capture of HG007 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500524
B-Lymphocyte
0.615
4.2
29990
3200.7
108
966.5
611
2136898.4
0.993
title: Methylome capture of HG007 using TrueMethyl oxidative bisulite sequencing, oxidative replicate; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500585
B-Lymphocyte
0.518
11.8
38940
15918.6
1209
1062.4
1576
1052474.8
0.997
title: Methylome capture of HG001 using EMSeq with 100ng of input DNA; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500586
B-Lymphocyte
0.518
11.7
39432
15874.5
1166
1068.9
1519
1079574.5
0.997
title: Methylome capture of HG001 using EMSeq with 100ng of input DNA; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500587
B-Lymphocyte
0.510
15.8
46185
13804.7
2189
1049.6
1470
1101367.0
0.998
title: Methylome capture of HG001 using EMSeq with 100ng of input DNA; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500589
B-Lymphocyte
0.522
13.4
44971
14533.4
1566
1060.9
1568
1057455.7
0.999
title: Methylome capture of HG001 using EMSeq with 100ng of input DNA; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500590
B-Lymphocyte
0.658
13.6
46549
2429.4
1309
938.8
661
1837158.6
0.997
title: Methylome capture of HG002 using EMSeq with 100ng of input DNA; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500591
B-Lymphocyte
0.659
12.7
45554
2425.3
1137
892.0
706
1759751.0
0.997
title: Methylome capture of HG002 using EMSeq with 100ng of input DNA; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500592
B-Lymphocyte
0.645
13.8
45840
2349.4
1623
896.8
748
1646361.6
0.998
title: Methylome capture of HG002 using EMSeq with 100ng of input DNA; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500593
B-Lymphocyte
0.643
13.7
45666
2358.6
1627
908.9
833
1535499.0
0.998
title: Methylome capture of HG002 using EMSeq with 100ng of input DNA; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500594
B-Lymphocyte
0.568
13.9
45176
10343.3
946
943.0
820
1683683.4
0.997
title: Methylome capture of HG003 using EMSeq with 100ng of input DNA; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500595
B-Lymphocyte
0.569
13.0
44094
10518.1
845
1003.7
823
1684900.6
0.997
title: Methylome capture of HG003 using EMSeq with 100ng of input DNA; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500596
B-Lymphocyte
0.559
13.4
43957
10130.5
1288
916.7
854
1620051.7
0.998
title: Methylome capture of HG003 using EMSeq with 100ng of input DNA; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500597
B-Lymphocyte
0.559
14.4
45063
9949.2
1422
942.0
842
1634568.0
0.998
title: Methylome capture of HG003 using EMSeq with 100ng of input DNA; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500598
B-Lymphocyte
0.616
11.1
47916
5974.0
1128
1138.4
682
1926602.3
0.997
title: Methylome capture of HG004 using EMSeq with 100ng of input DNA; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500599
B-Lymphocyte
0.642
15.3
45510
2717.4
889
917.1
598
2011798.6
0.998
title: Methylome capture of HG006 using EMSeq with 100ng of input DNA; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500600
B-Lymphocyte
0.662
13.5
44221
2812.8
1397
1072.4
588
2058821.4
0.995
title: Methylome capture of HG007 using EMSeq with 10ng of input DNA; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500602
B-Lymphocyte
0.656
12.9
44428
2762.2
1416
1061.8
682
1890105.0
0.997
title: Methylome capture of HG007 using EMSeq with 50ng of input DNA; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500603
B-Lymphocyte
0.653
12.7
44182
2730.2
1367
1068.2
616
2014621.4
0.997
title: Methylome capture of HG007 using EMSeq with 100ng of input DNA; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500604
B-Lymphocyte
0.641
24.7
48887
2860.6
2699
1025.5
721
1797844.2
0.997
title: Methylome capture of HG007 using EMSeq with 10ng of input DNA; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500605
B-Lymphocyte
0.649
15.7
45650
2707.0
1910
1040.2
654
1902303.2
0.998
title: Methylome capture of HG007 using EMSeq with 50ng of input DNA; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500607
B-Lymphocyte
0.644
15.9
44623
2740.0
1935
1042.8
613
1976587.1
0.998
title: Methylome capture of HG007 using EMSeq with 100ng of input DNA; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500620
B-Lymphocyte
0.520
3.3
8596
36756.9
36
1105.7
1434
1175933.7
0.983
title: Methylome capture of HG001 using Accel-NGS Methyl-Seq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500621
B-Lymphocyte
0.520
3.4
9018
36159.1
28
1250.9
1405
1192155.4
0.983
title: Methylome capture of HG001 using Accel-NGS Methyl-Seq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500622
B-Lymphocyte
0.523
12.4
39123
16782.0
1004
1076.8
1651
1019448.5
0.984
title: Methylome capture of HG001 using Accel-NGS Methyl-Seq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500623
B-Lymphocyte
0.526
2.9
8377
36047.2
19
1286.5
1334
1238857.1
0.984
title: Methylome capture of HG001 using Accel-NGS Methyl-Seq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500624
B-Lymphocyte
0.526
2.9
9174
34906.0
22
1295.8
1339
1235717.0
0.985
title: Methylome capture of HG001 using Accel-NGS Methyl-Seq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500626
B-Lymphocyte
0.528
10.8
37268
16706.2
794
1085.3
1543
1070215.9
0.985
title: Methylome capture of HG001 using Accel-NGS Methyl-Seq; {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500627
B-Lymphocyte
0.667
4.9
34965
2539.1
108
1035.8
641
2036319.2
0.984
title: Methylome capture of HG002 using Accel-NGS Methyl-Seq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500628
B-Lymphocyte
0.667
5.0
34406
2543.6
110
1126.1
657
2014603.3
0.984
title: Methylome capture of HG002 using Accel-NGS Methyl-Seq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500629
B-Lymphocyte
0.670
18.8
52103
2359.7
1624
893.0
759
1699450.6
0.984
title: Methylome capture of HG002 using Accel-NGS Methyl-Seq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500630
B-Lymphocyte
0.668
3.2
29634
2961.1
28
1434.4
636
2078180.6
0.984
title: Methylome capture of HG002 using Accel-NGS Methyl-Seq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500631
B-Lymphocyte
0.669
3.2
30146
2961.3
36
1090.4
649
2059118.6
0.984
title: Methylome capture of HG002 using Accel-NGS Methyl-Seq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500632
B-Lymphocyte
0.671
11.6
44904
2338.9
684
900.8
588
1984974.2
0.985
title: Methylome capture of HG002 using Accel-NGS Methyl-Seq; {"isolate": "NA24385", "age": "45", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500633
B-Lymphocyte
0.576
4.1
30552
14149.3
37
1232.6
987
1554396.9
0.984
title: Methylome capture of HG003 using Accel-NGS Methyl-Seq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500634
B-Lymphocyte
0.576
4.1
29715
14152.5
40
1200.6
1013
1523219.9
0.984
title: Methylome capture of HG003 using Accel-NGS Methyl-Seq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500635
B-Lymphocyte
0.578
15.8
48611
10429.8
1030
958.7
992
1498184.4
0.984
title: Methylome capture of HG003 using Accel-NGS Methyl-Seq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500637
B-Lymphocyte
0.576
4.3
30642
14239.5
59
1118.5
1082
1468490.5
0.984
title: Methylome capture of HG003 using Accel-NGS Methyl-Seq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500638
B-Lymphocyte
0.576
4.3
31083
14222.9
49
1132.1
1117
1429368.7
0.984
title: Methylome capture of HG003 using Accel-NGS Methyl-Seq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500639
B-Lymphocyte
0.578
15.2
46900
10250.2
848
953.9
952
1525579.3
0.984
title: Methylome capture of HG003 using Accel-NGS Methyl-Seq; {"isolate": "NA24149", "age": "90", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500640
B-Lymphocyte
0.628
4.7
38462
6367.5
124
1459.5
820
1801621.9
0.983
title: Methylome capture of HG004 using Accel-NGS Methyl-Seq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500641
B-Lymphocyte
0.629
4.8
39724
6388.4
137
1449.9
870
1738936.5
0.983
title: Methylome capture of HG004 using Accel-NGS Methyl-Seq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500642
B-Lymphocyte
0.631
17.5
52190
5582.0
1493
1107.5
727
1864515.6
0.983
title: Methylome capture of HG004 using Accel-NGS Methyl-Seq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500643
B-Lymphocyte
0.629
3.7
35961
6583.1
46
2234.3
825
1807256.9
0.984
title: Methylome capture of HG004 using Accel-NGS Methyl-Seq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500644
B-Lymphocyte
0.629
3.8
36260
6602.6
59
1889.8
840
1784306.7
0.984
title: Methylome capture of HG004 using Accel-NGS Methyl-Seq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500645
B-Lymphocyte
0.631
13.5
49429
5618.1
1115
1142.6
708
1884542.4
0.985
title: Methylome capture of HG004 using Accel-NGS Methyl-Seq; {"isolate": "NA24143", "age": "74", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500646
B-Lymphocyte
0.637
4.1
34671
5385.9
44
1221.1
986
1561275.1
0.983
title: Methylome capture of HG005 using Accel-NGS Methyl-Seq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500648
B-Lymphocyte
0.637
4.2
36627
5436.6
45
1237.4
992
1556751.7
0.983
title: Methylome capture of HG005 using Accel-NGS Methyl-Seq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500649
B-Lymphocyte
0.640
15.0
50893
5009.8
466
948.0
845
1657599.6
0.984
title: Methylome capture of HG005 using Accel-NGS Methyl-Seq; {"isolate": "NA24631", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500653
B-Lymphocyte
0.659
3.7
31135
3280.6
40
1142.4
785
1851529.4
0.984
title: Methylome capture of HG006 using Accel-NGS Methyl-Seq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500654
B-Lymphocyte
0.659
3.8
31215
3255.8
28
1311.0
756
1894629.5
0.984
title: Methylome capture of HG006 using Accel-NGS Methyl-Seq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500655
B-Lymphocyte
0.661
14.0
45952
2858.3
447
967.4
642
1977899.4
0.984
title: Methylome capture of HG006 using Accel-NGS Methyl-Seq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500656
B-Lymphocyte
0.659
4.1
32471
3236.4
34
1183.7
788
1846796.4
0.985
title: Methylome capture of HG006 using Accel-NGS Methyl-Seq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500657
B-Lymphocyte
0.659
4.1
32866
3219.2
44
1130.0
840
1765807.6
0.985
title: Methylome capture of HG006 using Accel-NGS Methyl-Seq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500659
B-Lymphocyte
0.662
14.9
46730
2862.1
464
962.9
621
2007402.9
0.985
title: Methylome capture of HG006 using Accel-NGS Methyl-Seq; {"isolate": "NA24694", "age": "NA", "biomaterial_provider": "NIST", "sex": "male", "tissue": "B-Lymphocyte"}
SRX9500660
B-Lymphocyte
0.665
2.9
28953
3324.3
20
1363.5
699
2015392.4
0.983
title: Methylome capture of HG007 using Accel-NGS Methyl-Seq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500661
B-Lymphocyte
0.665
2.9
29165
3314.7
24
1182.0
706
1992921.6
0.983
title: Methylome capture of HG007 using Accel-NGS Methyl-Seq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500662
B-Lymphocyte
0.666
14.8
46229
2837.4
1292
1050.6
715
1852253.7
0.984
title: Methylome capture of HG007 using Accel-NGS Methyl-Seq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500663
B-Lymphocyte
0.664
3.9
32268
3043.6
59
1137.9
823
1807058.5
0.983
title: Methylome capture of HG007 using Accel-NGS Methyl-Seq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500664
B-Lymphocyte
0.665
4.0
32070
3039.4
63
1082.0
839
1776733.7
0.984
title: Methylome capture of HG007 using Accel-NGS Methyl-Seq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500665
B-Lymphocyte
0.667
10.8
41931
2657.9
813
1059.2
595
2041138.2
0.983
title: Methylome capture of HG007 using Accel-NGS Methyl-Seq; {"isolate": "NA24695", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500668
B-Lymphocyte
0.530
4.0
10602
27077.9
125
1048.3
1303
1267427.8
0.996
title: Methylome capture of HG001 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
SRX9500670
B-Lymphocyte
0.533
3.4
9445
26577.0
93
1164.1
1269
1297210.0
0.996
title: Methylome capture of HG001 using SPlinted Ligation Adapter Tagging (SPLAT); {"isolate": "NA12878", "age": "NA", "biomaterial_provider": "NIST", "sex": "female", "tissue": "B-Lymphocyte"}
Methods
All analysis was done using a bisulfite sequnecing data analysis
pipeline DNMTools
developed in the Smith lab at USC.
Mapping reads from bisulfite sequencing :
Bisulfite treated reads are mapped to the genomes with the
abismal
program. Input reads are
filtered by their quality, and adapter sequences in the 3' end of
reads are trimmed. This is done with cutadapt. Uniquely mapped reads
with mismatches/indels below given threshold are retained. For
pair-end reads, if the two mates overlap, the overlapping part of the
mate with lower quality is discarded. After mapping, we use the format
command in dnmtools to merge mates for paired-end reads. We use the
dnmtools uniq command to randomly select one from multiple reads
mapped exactly to the same location. Without random oligos as UMIs,
this is our best indication of PCR duplicates.
Estimating methylation levels :
After reads are mapped and filtered, the
dnmtools counts
command is used to obtain read
coverage and estimate methylation levels at individual cytosine
sites. We count the number of methylated reads (those containing a
C) and the number of unmethylated reads (those containing a T) at
each nucleotide in a mapped read that corresponds to a cytosine in
the reference genome. The methylation level of that cytosine is
estimated as the ratio of methylated to total reads covering that
cytosine. For cytosines in the symmetric CpG sequence context,
reads from the both strands are collapsed to give a single
estimate. Very rarely do the levels differ between strands
(typically only if there has been a substitution, as in a somatic
mutation), and this approach gives a better estimate.
Bisulfite conversion rate :
The bisulfite conversion rate for an experiment is estimated with
the dnmtools bsrate
command, which computes the
fraction of successfully converted nucleotides in reads (those
read out as Ts) among all nucleotides in the reads mapped that map
over cytosines in the reference genome. This is done either using
a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear
genome. In the latter case, only non-CpG sites are used. While
this latter approach can be impacted by non-CpG cytosine
methylation, in practice it never amounts to much.
Identifying hypomethylated regions (HMRs) :
In most mammalian cells, the majority of the genome has high
methylation, and regions of low methylation are typically the
interesting features. (This seems to be true for essentially all
healthy differentiated cell types, but not cells of very early
embryogenesis, various germ cells and precursors, and placental
lineage cells.) These are valleys of low methylation are called
hypomethylated regions (HMR) for historical reasons. To identify
the HMRs, we use the dnmtools hmr
command, which uses
a statistical model that accounts for both the methylation level
fluctations and the varying amounts of data available at each CpG
site.
Partially methylated domains :
Partially methylated domains are large genomic regions showing
partial methylation observed in immortalized cell lines and
cancerous cells. The pmd
program is used to identify
PMDs.
Allele-specific methylation :
Allele-Specific methylated regions refers to regions where the
parental allele is differentially methylated compared to the
maternal allele. The program allelic
is used to
compute allele-specific methylation score can be computed for each
CpG site by testing the linkage between methylation status of
adjacent reads, and the program amrfinder
is used to
identify regions with allele-specific methylation.
For more detailed description of the methods of each step, please
refer to
the DNMTools
documentation .
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