Human methylome studies SRP126677 Track Settings
 
Genome-wide profiling at single-nucleotide resolution of brain cell types in schizophrenia [WGBS] [Brain]

Track collection: Human methylome studies

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experiment
SRX3468746 
SRX3468747 
SRX3468748 
SRX3468749 
SRX3468750 
SRX3468751 
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SRX3468753 
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SRX3468768 
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SRX3468800 
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SRX3468835 
SRX3468836 
experiment
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 SRX3468746  HMR  Brain / SRX3468746 (HMR)   Schema 
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 SRX3468746  CpG methylation  Brain / SRX3468746 (CpG methylation)   Schema 
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 SRX3468747  HMR  Brain / SRX3468747 (HMR)   Schema 
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 SRX3468747  CpG methylation  Brain / SRX3468747 (CpG methylation)   Schema 
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 SRX3468748  HMR  Brain / SRX3468748 (HMR)   Schema 
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 SRX3468748  CpG methylation  Brain / SRX3468748 (CpG methylation)   Schema 
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 SRX3468749  HMR  Brain / SRX3468749 (HMR)   Schema 
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 SRX3468749  CpG methylation  Brain / SRX3468749 (CpG methylation)   Schema 
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 SRX3468750  HMR  Brain / SRX3468750 (HMR)   Schema 
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 SRX3468750  CpG methylation  Brain / SRX3468750 (CpG methylation)   Schema 
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 SRX3468751  HMR  Brain / SRX3468751 (HMR)   Schema 
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 SRX3468751  CpG methylation  Brain / SRX3468751 (CpG methylation)   Schema 
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 SRX3468752  HMR  Brain / SRX3468752 (HMR)   Schema 
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 SRX3468752  CpG methylation  Brain / SRX3468752 (CpG methylation)   Schema 
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 SRX3468753  HMR  Brain / SRX3468753 (HMR)   Schema 
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 SRX3468753  CpG methylation  Brain / SRX3468753 (CpG methylation)   Schema 
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 SRX3468754  HMR  Brain / SRX3468754 (HMR)   Schema 
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 SRX3468754  CpG methylation  Brain / SRX3468754 (CpG methylation)   Schema 
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 SRX3468755  HMR  Brain / SRX3468755 (HMR)   Schema 
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 SRX3468755  CpG methylation  Brain / SRX3468755 (CpG methylation)   Schema 
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 SRX3468756  HMR  Brain / SRX3468756 (HMR)   Schema 
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 SRX3468756  CpG methylation  Brain / SRX3468756 (CpG methylation)   Schema 
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 SRX3468757  HMR  Brain / SRX3468757 (HMR)   Schema 
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 SRX3468757  CpG methylation  Brain / SRX3468757 (CpG methylation)   Schema 
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 SRX3468758  HMR  Brain / SRX3468758 (HMR)   Schema 
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 SRX3468758  CpG methylation  Brain / SRX3468758 (CpG methylation)   Schema 
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 SRX3468759  HMR  Brain / SRX3468759 (HMR)   Schema 
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 SRX3468759  CpG methylation  Brain / SRX3468759 (CpG methylation)   Schema 
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 SRX3468760  HMR  Brain / SRX3468760 (HMR)   Schema 
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 SRX3468760  CpG methylation  Brain / SRX3468760 (CpG methylation)   Schema 
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 SRX3468761  HMR  Brain / SRX3468761 (HMR)   Schema 
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 SRX3468761  CpG methylation  Brain / SRX3468761 (CpG methylation)   Schema 
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 SRX3468762  HMR  Brain / SRX3468762 (HMR)   Schema 
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 SRX3468762  CpG methylation  Brain / SRX3468762 (CpG methylation)   Schema 
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 SRX3468763  HMR  Brain / SRX3468763 (HMR)   Schema 
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 SRX3468763  CpG methylation  Brain / SRX3468763 (CpG methylation)   Schema 
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 SRX3468764  HMR  Brain / SRX3468764 (HMR)   Schema 
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 SRX3468764  CpG methylation  Brain / SRX3468764 (CpG methylation)   Schema 
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 SRX3468765  HMR  Brain / SRX3468765 (HMR)   Schema 
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 SRX3468765  CpG methylation  Brain / SRX3468765 (CpG methylation)   Schema 
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 SRX3468766  HMR  Brain / SRX3468766 (HMR)   Schema 
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 SRX3468766  CpG methylation  Brain / SRX3468766 (CpG methylation)   Schema 
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 SRX3468767  HMR  Brain / SRX3468767 (HMR)   Schema 
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 SRX3468767  CpG methylation  Brain / SRX3468767 (CpG methylation)   Schema 
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 SRX3468768  HMR  Brain / SRX3468768 (HMR)   Schema 
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 SRX3468768  CpG methylation  Brain / SRX3468768 (CpG methylation)   Schema 
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 SRX3468769  HMR  Brain / SRX3468769 (HMR)   Schema 
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 SRX3468769  CpG methylation  Brain / SRX3468769 (CpG methylation)   Schema 
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 SRX3468774  HMR  Brain / SRX3468774 (HMR)   Schema 
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 SRX3468774  CpG methylation  Brain / SRX3468774 (CpG methylation)   Schema 
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 SRX3468775  HMR  Brain / SRX3468775 (HMR)   Schema 
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 SRX3468775  CpG methylation  Brain / SRX3468775 (CpG methylation)   Schema 
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 SRX3468776  HMR  Brain / SRX3468776 (HMR)   Schema 
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 SRX3468776  CpG methylation  Brain / SRX3468776 (CpG methylation)   Schema 
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 SRX3468777  HMR  Brain / SRX3468777 (HMR)   Schema 
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 SRX3468777  CpG methylation  Brain / SRX3468777 (CpG methylation)   Schema 
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 SRX3468778  HMR  Brain / SRX3468778 (HMR)   Schema 
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 SRX3468778  CpG methylation  Brain / SRX3468778 (CpG methylation)   Schema 
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 SRX3468779  HMR  Brain / SRX3468779 (HMR)   Schema 
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 SRX3468779  CpG methylation  Brain / SRX3468779 (CpG methylation)   Schema 
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 SRX3468780  HMR  Brain / SRX3468780 (HMR)   Schema 
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 SRX3468780  CpG methylation  Brain / SRX3468780 (CpG methylation)   Schema 
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 SRX3468781  HMR  Brain / SRX3468781 (HMR)   Schema 
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 SRX3468781  CpG methylation  Brain / SRX3468781 (CpG methylation)   Schema 
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 SRX3468782  HMR  Brain / SRX3468782 (HMR)   Schema 
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 SRX3468782  CpG methylation  Brain / SRX3468782 (CpG methylation)   Schema 
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 SRX3468783  HMR  Brain / SRX3468783 (HMR)   Schema 
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 SRX3468783  CpG methylation  Brain / SRX3468783 (CpG methylation)   Schema 
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 SRX3468784  HMR  Brain / SRX3468784 (HMR)   Schema 
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 SRX3468784  CpG methylation  Brain / SRX3468784 (CpG methylation)   Schema 
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 SRX3468785  HMR  Brain / SRX3468785 (HMR)   Schema 
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 SRX3468785  CpG methylation  Brain / SRX3468785 (CpG methylation)   Schema 
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 SRX3468786  HMR  Brain / SRX3468786 (HMR)   Schema 
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 SRX3468786  CpG methylation  Brain / SRX3468786 (CpG methylation)   Schema 
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 SRX3468787  HMR  Brain / SRX3468787 (HMR)   Schema 
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 SRX3468787  CpG methylation  Brain / SRX3468787 (CpG methylation)   Schema 
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 SRX3468788  HMR  Brain / SRX3468788 (HMR)   Schema 
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 SRX3468788  CpG methylation  Brain / SRX3468788 (CpG methylation)   Schema 
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 SRX3468789  HMR  Brain / SRX3468789 (HMR)   Schema 
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 SRX3468789  CpG methylation  Brain / SRX3468789 (CpG methylation)   Schema 
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 SRX3468790  HMR  Brain / SRX3468790 (HMR)   Schema 
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 SRX3468790  CpG methylation  Brain / SRX3468790 (CpG methylation)   Schema 
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 SRX3468791  HMR  Brain / SRX3468791 (HMR)   Schema 
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 SRX3468791  CpG methylation  Brain / SRX3468791 (CpG methylation)   Schema 
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 SRX3468792  HMR  Brain / SRX3468792 (HMR)   Schema 
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 SRX3468792  CpG methylation  Brain / SRX3468792 (CpG methylation)   Schema 
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 SRX3468793  HMR  Brain / SRX3468793 (HMR)   Schema 
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 SRX3468793  CpG methylation  Brain / SRX3468793 (CpG methylation)   Schema 
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 SRX3468794  HMR  Brain / SRX3468794 (HMR)   Schema 
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 SRX3468794  CpG methylation  Brain / SRX3468794 (CpG methylation)   Schema 
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 SRX3468796  HMR  Brain / SRX3468796 (HMR)   Schema 
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 SRX3468796  CpG methylation  Brain / SRX3468796 (CpG methylation)   Schema 
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 SRX3468797  HMR  Brain / SRX3468797 (HMR)   Schema 
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 SRX3468797  CpG methylation  Brain / SRX3468797 (CpG methylation)   Schema 
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 SRX3468798  HMR  Brain / SRX3468798 (HMR)   Schema 
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 SRX3468798  CpG methylation  Brain / SRX3468798 (CpG methylation)   Schema 
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 SRX3468799  HMR  Brain / SRX3468799 (HMR)   Schema 
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 SRX3468799  CpG methylation  Brain / SRX3468799 (CpG methylation)   Schema 
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 SRX3468800  HMR  Brain / SRX3468800 (HMR)   Schema 
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 SRX3468800  CpG methylation  Brain / SRX3468800 (CpG methylation)   Schema 
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 SRX3468801  HMR  Brain / SRX3468801 (HMR)   Schema 
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 SRX3468801  CpG methylation  Brain / SRX3468801 (CpG methylation)   Schema 
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 SRX3468802  HMR  Brain / SRX3468802 (HMR)   Schema 
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 SRX3468802  CpG methylation  Brain / SRX3468802 (CpG methylation)   Schema 
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 SRX3468803  HMR  Brain / SRX3468803 (HMR)   Schema 
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 SRX3468803  CpG methylation  Brain / SRX3468803 (CpG methylation)   Schema 
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 SRX3468804  HMR  Brain / SRX3468804 (HMR)   Schema 
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 SRX3468804  CpG methylation  Brain / SRX3468804 (CpG methylation)   Schema 
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 SRX3468805  HMR  Brain / SRX3468805 (HMR)   Schema 
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 SRX3468805  CpG methylation  Brain / SRX3468805 (CpG methylation)   Schema 
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 SRX3468807  HMR  Brain / SRX3468807 (HMR)   Schema 
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 SRX3468807  CpG methylation  Brain / SRX3468807 (CpG methylation)   Schema 
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 SRX3468808  HMR  Brain / SRX3468808 (HMR)   Schema 
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 SRX3468808  CpG methylation  Brain / SRX3468808 (CpG methylation)   Schema 
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 SRX3468809  HMR  Brain / SRX3468809 (HMR)   Schema 
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 SRX3468809  CpG methylation  Brain / SRX3468809 (CpG methylation)   Schema 
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 SRX3468810  HMR  Brain / SRX3468810 (HMR)   Schema 
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 SRX3468810  CpG methylation  Brain / SRX3468810 (CpG methylation)   Schema 
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 SRX3468811  HMR  Brain / SRX3468811 (HMR)   Schema 
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 SRX3468811  CpG methylation  Brain / SRX3468811 (CpG methylation)   Schema 
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 SRX3468812  HMR  Brain / SRX3468812 (HMR)   Schema 
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 SRX3468812  CpG methylation  Brain / SRX3468812 (CpG methylation)   Schema 
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 SRX3468813  HMR  Brain / SRX3468813 (HMR)   Schema 
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 SRX3468813  CpG methylation  Brain / SRX3468813 (CpG methylation)   Schema 
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 SRX3468814  HMR  Brain / SRX3468814 (HMR)   Schema 
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 SRX3468814  CpG methylation  Brain / SRX3468814 (CpG methylation)   Schema 
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 SRX3468815  HMR  Brain / SRX3468815 (HMR)   Schema 
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 SRX3468815  CpG methylation  Brain / SRX3468815 (CpG methylation)   Schema 
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 SRX3468816  HMR  Brain / SRX3468816 (HMR)   Schema 
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 SRX3468816  CpG methylation  Brain / SRX3468816 (CpG methylation)   Schema 
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 SRX3468817  HMR  Brain / SRX3468817 (HMR)   Schema 
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 SRX3468817  CpG methylation  Brain / SRX3468817 (CpG methylation)   Schema 
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 SRX3468818  HMR  Brain / SRX3468818 (HMR)   Schema 
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 SRX3468818  CpG methylation  Brain / SRX3468818 (CpG methylation)   Schema 
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 SRX3468819  HMR  Brain / SRX3468819 (HMR)   Schema 
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 SRX3468819  CpG methylation  Brain / SRX3468819 (CpG methylation)   Schema 
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 SRX3468820  HMR  Brain / SRX3468820 (HMR)   Schema 
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 SRX3468820  CpG methylation  Brain / SRX3468820 (CpG methylation)   Schema 
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 SRX3468821  HMR  Brain / SRX3468821 (HMR)   Schema 
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 SRX3468821  CpG methylation  Brain / SRX3468821 (CpG methylation)   Schema 
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 SRX3468822  HMR  Brain / SRX3468822 (HMR)   Schema 
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 SRX3468822  CpG methylation  Brain / SRX3468822 (CpG methylation)   Schema 
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 SRX3468823  HMR  Brain / SRX3468823 (HMR)   Schema 
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 SRX3468823  CpG methylation  Brain / SRX3468823 (CpG methylation)   Schema 
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 SRX3468824  HMR  Brain / SRX3468824 (HMR)   Schema 
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 SRX3468824  CpG methylation  Brain / SRX3468824 (CpG methylation)   Schema 
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 SRX3468825  HMR  Brain / SRX3468825 (HMR)   Schema 
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 SRX3468825  CpG methylation  Brain / SRX3468825 (CpG methylation)   Schema 
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 SRX3468826  HMR  Brain / SRX3468826 (HMR)   Schema 
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 SRX3468826  CpG methylation  Brain / SRX3468826 (CpG methylation)   Schema 
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 SRX3468827  HMR  Brain / SRX3468827 (HMR)   Schema 
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 SRX3468827  CpG methylation  Brain / SRX3468827 (CpG methylation)   Schema 
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 SRX3468828  HMR  Brain / SRX3468828 (HMR)   Schema 
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 SRX3468828  CpG methylation  Brain / SRX3468828 (CpG methylation)   Schema 
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 SRX3468829  HMR  Brain / SRX3468829 (HMR)   Schema 
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 SRX3468829  CpG methylation  Brain / SRX3468829 (CpG methylation)   Schema 
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 SRX3468830  HMR  Brain / SRX3468830 (HMR)   Schema 
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 SRX3468830  CpG methylation  Brain / SRX3468830 (CpG methylation)   Schema 
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 SRX3468832  HMR  Brain / SRX3468832 (HMR)   Schema 
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 SRX3468832  CpG methylation  Brain / SRX3468832 (CpG methylation)   Schema 
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 SRX3468833  HMR  Brain / SRX3468833 (HMR)   Schema 
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 SRX3468833  CpG methylation  Brain / SRX3468833 (CpG methylation)   Schema 
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 SRX3468834  HMR  Brain / SRX3468834 (HMR)   Schema 
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 SRX3468834  CpG methylation  Brain / SRX3468834 (CpG methylation)   Schema 
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 SRX3468835  HMR  Brain / SRX3468835 (HMR)   Schema 
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 SRX3468835  CpG methylation  Brain / SRX3468835 (CpG methylation)   Schema 
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 SRX3468836  HMR  Brain / SRX3468836 (HMR)   Schema 
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 SRX3468836  CpG methylation  Brain / SRX3468836 (CpG methylation)   Schema 
    

Study title: Genome-wide profiling at single-nucleotide resolution of brain cell types in schizophrenia [WGBS]
SRA: SRP126677
GEO: GSE107729
Pubmed: 31288836

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Details
SRX3468746 Brain 0.805 17.7 63875 1406.8 11108 994.9 5366 21670.0 0.950 title: GSM2877162 1535_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1535; age: 52; disease: Control; sex: male
SRX3468747 Brain 0.744 14.5 53131 1268.8 10236 2695.9 4581 18612.8 0.953 title: GSM2877163 1516_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1516; age: 47; disease: Schizo; sex: male
SRX3468748 Brain 0.798 13.4 54021 1283.7 6828 949.9 4994 20150.5 0.952 title: GSM2877164 1509_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1509; age: 52; disease: Schizo; sex: male
SRX3468749 Brain 0.797 15.6 55355 1258.7 7204 3421.6 4951 20912.2 0.960 title: GSM2877165 1519_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1519; age: 54; disease: Schizo; sex: male
SRX3468750 Brain 0.812 13.6 56580 1324.9 6972 945.8 5420 20776.5 0.950 title: GSM2877166 1533_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1533; age: 54; disease: Control; sex: male
SRX3468751 Brain 0.780 17.0 53202 1288.2 12205 1011.3 4377 20423.0 0.962 title: GSM2877167 1537_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1537; age: 46; disease: Control; sex: male
SRX3468752 Brain 0.824 29.0 72408 1628.3 9875 940.0 6521 22353.2 0.943 title: GSM2877168 1517_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1517; age: 77; disease: Schizo; sex: male
SRX3468753 Brain 0.785 34.0 68840 1489.1 7388 937.7 5107 20145.1 0.943 title: GSM2877169 1531_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1531; age: 60; disease: Control; sex: male
SRX3468754 Brain 0.763 30.8 71138 1515.7 7249 969.9 5670 19590.9 0.949 title: GSM2877170 1534_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1534; age: 75; disease: Control; sex: male
SRX3468755 Brain 0.810 30.1 65144 1653.0 14231 1025.6 4932 52028.2 0.953 title: GSM2877171 1512_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1512; age: 43; disease: Schizo; sex: female
SRX3468756 Brain 0.785 26.3 77970 1332.0 1609 1025.0 4280 43131.0 0.989 title: GSM2877172 1512_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1512; age: 43; disease: Schizo; sex: female
SRX3468757 Brain 0.779 28.7 79921 1308.4 1338 1042.5 4417 43396.4 0.989 title: GSM2877173 1523_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1523; age: 42; disease: Schizo; sex: female
SRX3468758 Brain 0.797 25.5 60876 1454.0 14108 1017.3 4883 22259.2 0.951 title: GSM2877174 1524_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1524; age: 40; disease: Control; sex: male
SRX3468759 Brain 0.816 32.5 71976 1693.5 13194 992.6 5138 48462.7 0.934 title: GSM2877175 4336_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 4336; age: 85; disease: Schizo; sex: female
SRX3468760 Brain 0.830 34.5 60168 1802.8 20889 1090.1 4706 29311.6 0.904 title: GSM2877176 3545_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 3545; age: 80; disease: Control; sex: male
SRX3468761 Brain 0.799 39.0 77506 1478.2 1508 901.5 4079 30692.7 0.977 title: GSM2877177 3545_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 3545; age: 80; disease: Control; sex: male
SRX3468762 Brain 0.828 38.1 76011 1736.7 13085 978.0 6481 24589.6 0.927 title: GSM2877178 3586_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 3586; age: 76; disease: Control; sex: male
SRX3468763 Brain 0.810 33.0 74245 1860.7 13141 1012.1 4503 63060.4 0.952 title: GSM2877179 1505_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1505; age: 46; disease: Schizo; sex: female
SRX3468764 Brain 0.800 37.0 69350 1463.5 5679 972.1 4251 46550.6 0.986 title: GSM2877180 1505_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1505; age: 46; disease: Schizo; sex: female
SRX3468765 Brain 0.807 28.9 68697 1613.3 13089 992.3 4714 55203.6 0.947 title: GSM2877181 1523_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1523; age: 42; disease: Schizo; sex: female
SRX3468766 Brain 0.793 27.9 80805 1287.1 1098 1079.8 4361 43451.7 0.987 title: GSM2877182 4336_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 4336; age: 85; disease: Schizo; sex: female
SRX3468767 Brain 0.785 23.1 76574 1229.4 2605 912.4 4402 22789.5 0.990 title: GSM2877183 1524_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1524; age: 40; disease: Control; sex: male
SRX3468768 Brain 0.798 34.5 84275 1357.9 820 893.9 4319 28170.0 0.987 title: GSM2877184 3586_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 3586; age: 76; disease: Control; sex: male
SRX3468769 Brain 0.815 30.1 74587 1717.8 11127 977.4 4897 55548.9 0.943 title: GSM2877185 4361_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 4361; age: 77; disease: Schizo; sex: female
SRX3468774 Brain 0.808 39.1 78284 1414.6 1024 891.2 3921 31596.7 0.983 title: GSM2877190 1507_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1507; age: 32; disease: Schizo; sex: male
SRX3468775 Brain 0.808 25.1 69766 1626.8 12560 988.6 4258 61643.0 0.954 title: GSM2877191 1508_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1508; age: 73; disease: Schizo; sex: female
SRX3468776 Brain 0.820 25.3 66333 1625.4 14229 1018.9 5210 25462.9 0.950 title: GSM2877192 1510_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1510; age: 33; disease: Schizo; sex: male
SRX3468777 Brain 0.821 28.5 73799 1692.3 13301 996.8 6047 27861.2 0.949 title: GSM2877193 1511_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1511; age: 26; disease: Schizo; sex: male
SRX3468778 Brain 0.821 33.0 67034 1735.3 14955 1033.4 4622 54167.3 0.952 title: GSM2877194 1513_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1513; age: 73; disease: Schizo; sex: female
SRX3468779 Brain 0.820 36.5 67174 1732.9 16511 1042.2 5726 24724.0 0.953 title: GSM2877195 1514_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1514; age: 40; disease: Schizo; sex: male
SRX3468780 Brain 0.821 34.9 69022 1798.0 13706 1007.5 3998 65705.7 0.953 title: GSM2877196 1515_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1515; age: 50; disease: Schizo; sex: female
SRX3468781 Brain 0.817 33.5 71848 1795.6 12863 994.1 4740 57699.6 0.947 title: GSM2877197 1518_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1518; age: 52; disease: Schizo; sex: female
SRX3468782 Brain 0.814 33.4 69508 1808.8 14712 1020.9 4460 59770.0 0.950 title: GSM2877198 1525_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1525; age: 65; disease: Control; sex: female
SRX3468783 Brain 0.803 25.4 79251 1300.3 663 1000.4 3773 30012.2 0.991 title: GSM2877199 1511_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1511; age: 26; disease: Schizo; sex: male
SRX3468784 Brain 0.808 31.2 72010 1770.5 13536 997.4 5759 28797.8 0.950 title: GSM2877200 1527_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1527; age: 36; disease: Control; sex: male
SRX3468785 Brain 0.805 35.4 73770 1783.2 14035 1003.8 4600 57692.0 0.936 title: GSM2877201 1532_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1532; age: 68; disease: Control; sex: female
SRX3468786 Brain 0.808 30.4 70695 1646.8 13774 996.2 5794 24990.3 0.948 title: GSM2877202 1536_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1536; age: 41; disease: Control; sex: male
SRX3468787 Brain 0.809 35.1 73704 1700.6 12695 988.7 5051 50227.1 0.944 title: GSM2877203 1538_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1538; age: 55; disease: Control; sex: female
SRX3468788 Brain 0.808 32.7 69674 1764.5 14073 1006.1 4288 64452.6 0.950 title: GSM2877204 1539_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1539; age: 53; disease: Control; sex: female
SRX3468789 Brain 0.810 35.0 72658 1728.4 14789 1017.4 5368 48134.7 0.931 title: GSM2877205 1541_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1541; age: 49; disease: Control; sex: female
SRX3468790 Brain 0.799 40.6 65274 1858.3 19158 1076.3 5240 26513.2 0.951 title: GSM2877206 3602_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 3602; age: 66; disease: Control; sex: male
SRX3468791 Brain 0.819 37.4 68501 1694.3 17095 1014.6 5147 24784.8 0.951 title: GSM2877207 3611_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 3611; age: 64; disease: Control; sex: male
SRX3468792 Brain 0.818 34.9 58519 1654.9 15173 1019.3 4166 55952.5 0.944 title: GSM2877208 AN03398_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN03398; age: 75; disease: Control; sex: female
SRX3468793 Brain 0.805 33.3 78125 1763.6 15157 1018.0 5279 48722.4 0.922 title: GSM2877209 AN15240_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN15240; age: 36; disease: Control; sex: female
SRX3468794 Brain 0.834 46.6 79170 2210.3 31195 1197.6 5469 31179.5 0.924 title: GSM2877210 AN16799_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN16799; age: 43; disease: Control; sex: male
SRX3468796 Brain 0.798 37.4 78557 1385.8 831 896.6 4032 28765.3 0.991 title: GSM2877212 4615_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 4615; age: 49; disease: Control; sex: male
SRX3468797 Brain 0.825 32.6 69534 1624.1 13539 982.9 6308 21486.7 0.947 title: GSM2877213 AN05483_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN05483; age: 66; disease: Control; sex: male
SRX3468798 Brain 0.797 33.5 84063 1253.0 720 939.6 4265 24780.6 0.991 title: GSM2877214 AN05483_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: AN05483; age: 66; disease: Control; sex: male
SRX3468799 Brain 0.810 35.6 72628 1763.5 15049 1006.0 6310 24577.0 0.945 title: GSM2877215 AN10090_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN10090; age: 52; disease: Control; sex: male
SRX3468800 Brain 0.794 39.1 78730 1433.8 1429 1086.4 4557 41342.1 0.991 title: GSM2877216 1532_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1532; age: 68; disease: Control; sex: female
SRX3468801 Brain 0.790 37.2 78983 1448.4 1634 1075.2 4367 47139.2 0.991 title: GSM2877217 1539_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1539; age: 53; disease: Control; sex: female
SRX3468802 Brain 0.791 28.3 87030 1238.4 511 927.3 3193 33580.7 0.990 title: GSM2877218 AN10090_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: AN10090; age: 52; disease: Control; sex: male
SRX3468803 Brain 0.787 43.2 80969 1479.2 1106 900.9 3953 30734.7 0.992 title: GSM2877219 1527_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1527; age: 36; disease: Control; sex: male
SRX3468804 Brain 0.802 25.7 83674 1279.0 483 946.1 4011 30137.9 0.981 title: GSM2877220 1536_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1536; age: 41; disease: Control; sex: male
SRX3468805 Brain 0.789 38.2 76258 1389.0 2857 1003.0 5132 33057.0 0.990 title: GSM2877221 1538_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1538; age: 55; disease: Control; sex: female
SRX3468807 Brain 0.839 25.8 63567 1612.6 10663 961.8 5546 25719.5 0.939 title: GSM2877223 4395_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 4395; age: 56; disease: Schizo; sex: male
SRX3468808 Brain 0.798 33.3 81835 1332.0 709 923.7 4329 28335.4 0.989 title: GSM2877224 4395_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 4395; age: 56; disease: Schizo; sex: male
SRX3468809 Brain 0.801 22.4 70541 1571.7 13915 1006.0 5339 25185.1 0.916 title: GSM2877225 4504_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 4504; age: 55; disease: Schizo; sex: male
SRX3468810 Brain 0.784 27.9 78557 1365.9 1521 1101.8 4197 46844.7 0.991 title: GSM2877226 AN17799_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: AN17799; age: 56; disease: Schizo; sex: female
SRX3468811 Brain 0.815 40.8 71070 1840.7 14465 997.3 5743 28026.4 0.947 title: GSM2877227 4448_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 4448; age: 46; disease: Schizo; sex: male
SRX3468812 Brain 0.821 34.6 67713 1636.0 13236 987.1 5801 23784.4 0.948 title: GSM2877228 3590_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 3590; age: 75; disease: Control; sex: male
SRX3468813 Brain 0.807 30.5 78860 1465.2 10871 1081.2 3866 32079.1 0.930 title: GSM2877229 3590_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 3590; age: 75; disease: Control; sex: male
SRX3468814 Brain 0.800 42.0 73579 1469.5 4547 941.4 4137 29746.0 0.980 title: GSM2877230 1520_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1520; age: 38; disease: Schizo; sex: male
SRX3468815 Brain 0.795 37.5 83283 1495.9 1435 1036.2 3683 57648.9 0.976 title: GSM2877231 1525_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1525; age: 65; disease: Control; sex: female
SRX3468816 Brain 0.809 32.1 72650 1830.2 14217 1016.2 4592 59850.0 0.950 title: GSM2877232 AN17799_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN17799; age: 56; disease: Schizo; sex: female
SRX3468817 Brain 0.800 37.7 77852 1483.9 1976 885.0 3975 31473.9 0.970 title: GSM2877233 4448_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 4448; age: 46; disease: Schizo; sex: male
SRX3468818 Brain 0.792 37.3 76624 1341.3 789 936.5 3904 27876.4 0.990 title: GSM2877234 4504_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 4504; age: 55; disease: Schizo; sex: male
SRX3468819 Brain 0.801 42.7 80250 1437.9 1068 903.0 4229 27819.7 0.990 title: GSM2877235 3602_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 3602; age: 66; disease: Control; sex: male
SRX3468820 Brain 0.813 43.0 82240 1426.0 5222 971.3 4043 29982.2 0.942 title: GSM2877236 3611_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 3611; age: 64; disease: Control; sex: male
SRX3468821 Brain 0.791 46.0 78546 1532.0 1054 916.0 3822 31969.0 0.989 title: GSM2877237 4804_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 4804; age: 61; disease: Schizo; sex: male
SRX3468822 Brain 0.793 43.9 80480 1495.5 1635 1060.0 4927 40842.7 0.991 title: GSM2877238 AN03398_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: AN03398; age: 75; disease: Control; sex: female
SRX3468823 Brain 0.785 44.2 79347 1569.2 1706 1071.6 4241 47315.1 0.989 title: GSM2877239 AN15240_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: AN15240; age: 36; disease: Control; sex: female
SRX3468824 Brain 0.783 48.1 82933 1586.4 3501 873.7 3998 29610.8 0.989 title: GSM2877240 AN16799_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: AN16799; age: 43; disease: Control; sex: male
SRX3468825 Brain 0.799 43.7 82802 1785.7 9136 951.3 6588 25334.9 0.947 title: GSM2877241 4804_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 4804; age: 61; disease: Schizo; sex: male
SRX3468826 Brain 0.795 43.8 81717 1358.9 809 940.3 3776 28084.1 0.986 title: GSM2877242 Miami0001_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: Miami0001; age: 25; disease: Control; sex: male
SRX3468827 Brain 0.819 38.5 64824 1723.4 14557 1009.1 5491 26953.8 0.955 title: GSM2877243 AN09634_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN09634; age: 26; disease: Schizo; sex: male
SRX3468828 Brain 0.811 39.3 71366 1783.3 13228 1001.8 5181 48627.2 0.950 title: GSM2877244 4730_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 4730; age: 32; disease: Schizo; sex: female
SRX3468829 Brain 0.798 29.6 57182 1481.3 11424 961.3 4030 26465.1 0.965 title: GSM2877245 AN18099_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: AN18099; age: 66; disease: Schizo; sex: male
SRX3468830 Brain 0.813 29.6 71166 1511.9 8888 941.2 6174 21246.8 0.948 title: GSM2877246 1521_WGBS_NeuN, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: NeuN; subject id: 1521; age: 60; disease: Schizo; sex: male
SRX3468832 Brain 0.795 35.4 79484 1435.7 1716 1052.5 4720 42436.5 0.990 title: GSM2877248 1513_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1513; age: 73; disease: Schizo; sex: female
SRX3468833 Brain 0.787 38.7 77852 1362.3 1456 920.4 4204 24851.5 0.988 title: GSM2877249 1514_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1514; age: 40; disease: Schizo; sex: male
SRX3468834 Brain 0.794 34.6 80180 1414.0 1072 1098.9 4015 52168.5 0.989 title: GSM2877250 1515_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1515; age: 50; disease: Schizo; sex: female
SRX3468835 Brain 0.780 35.6 81198 1450.0 1382 1111.4 3929 53264.2 0.989 title: GSM2877251 1518_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: 1518; age: 52; disease: Schizo; sex: female
SRX3468836 Brain 0.806 34.2 78103 1391.6 527 1022.0 3925 30700.8 0.989 title: GSM2877252 AN18099_WGBS_Olig2, Homo sapiens, Bisulfite-Seq; source_name: Brain BA46; tissue: brain; brain region: Brodmann Area 46 (BA46); cell_type: Olig2; subject id: AN18099; age: 66; disease: Schizo; sex: male

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.